Quantitative proteomic analysis to understand the role of Arabidopsis thaliana LEAFY COTYLEDON 2 in promoting lipid accumulation in Chlorella sorokiniana by upregulating photosynthetic proteins and G3PDH

Microalgae have attracted significant attention for biofuel production and transcription factors that can effectively regulate lipid accumulation in higher plants. In this study we expressed the factor LEAFY COTYLEDON 2 from Arabidopsis thaliana Chlorella sorokiniana. The relative electron transport rate (rel.ETR) of C. sorokiniana-AtLEC2 improved significantly under high-intensity light. As a result, total content main fatty acid composition (C16, C18) increased by more than onefold. Differentially proteins were analyzed using label-free strategy to elucidate regulation mechanism AtLEC2. results showed upregulation TAG biosynthesis (G3PDH) photosynthesis-related (e.g., ATP synthase F0, Photosystem II, I P700, cytochrome b6, b6f, chlorophyll a-b binding protein, assembly protein). Furthermore, AtLEC2 expression sorokiniana led fixed carbon flow photosynthesis through G3PDH upregulation. current will provide new direction cultivation high-yield oil algae reveal on